Research Papers

Attempting to synthesize lasso peptides using high pressure

by Mateusz Waliczek, Magdalena Wierzbicka, Maciej Arkuszewski, Monika Kijewska, Łukasz Jaremko, Priyadharshni Rajagopal, Kacper Szczepski, Amanda Sroczyńska, Mariusz Jaremko, Piotr Stefanowicz
PLOS ONE Year: 2020 DOI: 10.1371/journal.pone.0234901

Extra Information

Research article


Lasso peptides are unique in that the tail of the lasso peptide threads through its macrolactam ring. The unusual structure and biological activity of lasso peptides have generated increased interest from the scientific community in recent years. Because of this, many new types of lasso peptides have been discovered. These peptides can be synthesized by microorganisms efficiently, and yet, their chemical assembly is challenging. Herein, we investigated the possibility of high pressure inducing the cyclization of linear precursors of lasso peptides. Unlike other molecules like rotaxanes which mechanically interlock at high pressure, the threaded lasso peptides did not form, even at pressures the high pressure up to 14 000 kbar.